Diagnosis of mutations by the PCR double RFLP method (PCR-dRFLP)

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Diagnosis of mutations by the PCR double RFLP method (PCR-dRFLP).

The diagnosis of mutations that are responsible for human genetic diseases is important, and several molecular biology techniques based on polymerase chain reaction (PCR) (1), have been specially developed for this purpose (2-8) . For some of these techniques it is the presence or the absence of a PCR product that constitutes the analytical step, ie proves the presence of the mutation. In order...

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Leptospirosis is still one of the most important health problems in developing countries located in humid tropical and subtropical regions. Human infections are generally caused by exposure to water, soil or food contaminated with the urine of infected wild and domestic animals such as rodents and dogs. The clinical course of leptospirosis is variable and may be difficult to distinguish from ma...

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Identification of Mycobacteria species in cutaneous lesions of Sarcoidosis by PCR-Restriction Ragment Length Polymorphism (PCR-RFLP) method

Background: Sarcoidosis is a granulomatous multisystem disease of unknown etiology. It has recently been tired to detect Mycobacteria genome in biopsy specimens of patients with sarcoidosis by Polymorphism chain reaction method. Objective: To detect and identify Mycobacteria species in cutaneous lesions of the patients with sarcoidosis by PCR-RFLP. Patients and methods: 20 patients with clinica...

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A New PCR-RFLP Method for Diagnosing PNPLA3 rs738409 Polymorphism

Article ID: WMC002401 Article Type: Research Protocol Submitted on:01-Nov-2011, 12:31:07 PM GMT Published on: 02-Nov-2011, 05:42:47 PM GMT Article URL: http://www.webmedcentral.com/article_view/2401 Subject Categories:GENETICS

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1994

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/22.25.5773